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More Enzymatic Cleaners to the Rescue!

If you read our article “Breaking down Mycotoxins and mVOCs with Enzymes and Non-Toxic Cleaners”, we described that certain enzymes can be used to break down mycotoxins, the toxic products of mold, which can otherwise be very hard to eliminate.  Enzymatic cleaners specifically targeting mold and mycotoxins are few, but we wanted to let you know that all-purpose enzymatic cleaners work on stains, odors, and general cleaning duties all around the house!

First of all: What is an enzyme?   Enzymes are proteins produced by living organisms that act as catalysts in chemical reactions.  Enzymes can either build up or break down.  For our purposes, cleaning enzymes facilitate breaking down microbes and their byproducts that cause sickness, stinkiness or stains.  

Scientists have been exploring making artificial enzymes since the 1990’s, and many of these are mimicking enzymes found in nature.  Here are the most common types of natural enzymes (from Simple Science: How in the World do Enzymes Clean?):

• Proteases break down protein-based soils including blood, urine, food, feces, wine and other beverages.

• Lipases break down fat molecules like oils and grease.

• Amylases break down starch molecules like eggs, sugars, sauces, ice cream, gravy.

• Cellulases are used to soften fabric and restore color to fibers made up of cellulose material. They also remove particulate soil and reduce fabric graying and pilling.

Enzymes were initially produced by extraction from glands of various animals; however, modern enzyme production is done through fermentation of various fungi and bacteria through the steps of fermentation, recovery, and standardization.   (about Cleaning Products: Enzyme Science) 

Enzymes and purely enzymatic cleaners are not “alive”.  Bio-enzymatic cleaners, however, do combine enzymes with bacteria, which are “alive”. Examples are drain cleaners and some pet stain removers.  

Enzymatic cleaners are marketed to pet owners (and even more so to cat owners) because cat urine can be especially concentrated (cats make the most of the little water they drink), and so once it’s deposited, the ammonia and hormones in the urine start to smell, and bacteria start breaking it down into urea.  It’s an unmistakable fragrance, and enzymatic cleaners are regularly recommended by veterinarian and pet experts for pet accident cleanup (the ASPCA, mobile vet company The Vets, and professional veterinary site DVM360 are just a few examples).  

Some of the benefits of enzymatic cleaners are:

• They tend to be more powerful by working on more substrates (surfaces, conditions) in lower concentration than chemical cleaners.  Therefore you use less, resulting in less packaging.

• They work at moderate pH and temperature, allowing for milder detergents and less energy, like cold-water laundry detergents.

• Enzymes are not mutagenic (causing changes in DNA) and not clastogenic (causing breaks in chromosomes).  They are not reproductive or developmental toxins and have a low toxicity to aquatic systems.

Some of the cons of enzymatic cleaners are:

• They can have storage time limitations before the enzymes become less active.

• They can have storage temperature limitations.

• They can take longer to work.

• Some enzymatic cleaners (especially carpet cleaners as in this video) can leave an oily sticky residue if the cleaner is not washed away, which can attract soil/stain even more.

• They can have inhalation dangers if the cleaner is in powder form, but many formulations are now in dust-free granules that avoid this danger.

• They can be incompatible with other cleaners like bleach and chemical disinfectants, which can deactivate the enzymes.

So, here are some enzyme cleaners you might like to try:

For pet owners:  Rocco & Roxie Stain & Odor Eliminator for Strong Odor - Enzyme Pet Odor Eliminator for Home has a LOT of fans for the way it quickly and thoroughly eliminates pet odors and stains (just read some of the comments/watch videos).  Although their ingredients are a bit mysterious (Water, advanced biological blend, non-ionic surfactant, odor counteractant), the company says it is safe to use around pets and children, and certified safe for all carpets from the Carpet and Rug Institute (CRI).  They also offer a 100% money-back guarantee if you’re not satisfied.

For anyone who cleans: TweetMint Enzyme Cleaner contains no volatile organic compounds (VOC) or synthetic chemicals. It's completely free of artificial colors & fragrances, preservatives, animal products or by-products and has not been tested on animals.  Since it’s concentrated, a little (just ½ oz) will make ½ gallon of medium-strength concentrate, and the website publishes a whole list of jobs like cleaning windows and floors, degreasing, stain removal, odor elimination, and even pest control (garden and home).  The active ingredients are purified water, anionic/nonionic surfactant blend, glycerin, enzymes, peppermint oil, sodium borate. Sodium borate is a naturally-occurring compound made of boron, oxygen, hydrogen, and sodium, and in 2020 the Cosmetic Ingredient Review (CIR) Expert Panel evaluated data and concluded that sodium borate is safe as a cosmetic ingredients in concentrations less than or equal to 5%.. (Sodium Borate: Is it Safe?)

For anyone who showers or washes clothes:  Zerotaboos (ok, just put a space in there to easily say it: zero taboos!) is a woman-owned company which uses prebiotics (simple sugars, which are food for beneficial bacteria) and postbiotics (Lactobacilli ferment filtrate) in their products to encourage growth of good bacteria so that you don’t need harsh chemicals and fragrances to smell better.  Their Laundry Enzymes contain 10% nuclease enzymes, the strongest concentration available and the best for breaking down body odor.  It doesn’t replace your regular laundry detergent, but does a good job of removing troublesome body odor from clothing so that it actually smells fresh again after washing!  This owner knows her stuff and cares about her customers (see video on why she stopped offering refill packaging). For sure, bacteria and their byproducts have fed odors and fears long enough…it’s time to turn the tables by putting good bacteria and enzymes to work for us!

What are xenobiotics and POPs and how do our bodies deal with them?

Xenobiotics surround us everyday!  If you have an illness that you can pinpoint to a chemical or environmental exposure, then you know what a xenobiotic is and how it can seriously affect your health.     

• Xenobiotics have been defined as chemicals to which an organism is exposed that are extrinsic to the normal metabolism of that organism. (Progress in Molecular Biology and Translational Science).  Since mold produces mycotoxins that are not made in our own bodies, these mycotoxins are xenobiotics to us, as are many man-made chemicals like POPs. (Alcohol is also a xenobiotic).

• Persistent Organic Pollutants (POPs) are chemicals of global concern due to their potential for long-range transport, persistence in the environment, ability to bio-magnify and bio-accumulate in ecosystems, as well as their significant negative effects on human health and the environment. The most commonly encountered POPs are organochlorine pesticides, such as DDT, industrial chemicals, polychlorinated biphenyls (PCB) as well as unintentional by-products of many industrial processes, especially polychlorinated dibenzo-p-dioxins (PCDD) and dibenzofurans (PCDF), commonly known as dioxins. (Food safety: Persistent organic pollutants (POPs))  POPs are fat-soluable, and tend to accumulate in our fat tissues. POPs are xenobiotics, but not all xenobiotics are POPs. Exposure to POPs has been associated with diabetes, cardiovascular diseases and many other chronic diseases. (Glutathione!)  

Most of these xenobiotics are transformed by enzymes in the liver, and are then eliminated by excretion.  First of all: What is an enzyme?   Enzymes are complex proteins produced by living organisms that act as catalysts in chemical reactions.  Enzymes can either build up or break down.  Enzymes themselves are not consumed.

This is where our genes come in.  “GST” genes are important for detoxification of the body, in that they manufacture those enzymes that facilitate the detoxification reaction.  One of the most important GST enzymes is GSTP1.  

The GST pi gene encodes (provides instructions for building) the enzyme Glutathione S‑transferase Pi (GSTP1), which plays an important regulatory role in detoxification, anti‑oxidative damage, and the occurrence of various diseases.  The detoxification reaction is called “glutathione conjugation”.  (GSTP1 and cancer: Expression, methylation, polymorphisms and signaling (Review))  Following is an example of glutathione conjugation; note that the “SH” site on glutathione (sulfur) is the binding site for the xenobiotic:

Source: The role of glutathione-S-transferase in anti-cancer drug resistance

GSTP1 has a wide range of physiological functions: It is involved in metabolism, detoxification and elimination of potentially genotoxic foreign complexes, metabolizes a variety of carcinogenic compounds, and protects cells against DNA damage and canceration.  However, while GST mediates detoxification from accidental xenobiotics, like exposures to pesticide for example, GSTs have also been implicated in the development of resistance toward chemotherapy agents, especially platinum-based chemotherapy drugs. (GSTP1 and cancer: Expression, methylation, polymorphisms and signaling (Review))

Here are several more genetic terms that will help to understand how GST and GSTP1 work: 

• Gene expression is how GST directs the manufacture of GSTP1 (for more on how gene expression works, check out this article)

• Methylation is a chemical modification of DNA and other molecules that may be retained as cells divide to make more cells. When found in DNA, methylation can alter gene expression. In this process, chemical tags called methyl groups attach to a particular location within DNA where they turn a gene on or off, thereby regulating the production of proteins that the gene encodes. (National Human Genome Research Institute)

• Polymorphism, as related to genomics, refers to the presence of two or more variant forms of a specific DNA sequence that can occur among different individuals or populations. The most common type of polymorphism involves variation at a single nucleotide (also called a single-nucleotide polymorphism, or SNP). Other polymorphisms can be much larger, involving longer stretches of DNA. (National Human Genome Research Institute)

GSTP1 methylation can affect gene expression, inactivating the GST gene.  GSTP1 methylation has been associated with the development or recurrence of prostate cancer (PCa), liver and breast cancers. 

In addition, during detoxification of xenobiotics, GSTP1 may become damaged, causing polymorphism (a variation).  Some polymorphisms are associated with specific cancer types. For example, the genetic polymorphism of GSTP1 may be associated with the detoxification of polycyclic aromatic hydrocarbons (PAHs) in cigarette smoke and exhibits the highest expression in lung tissue.  More polymorphisms of GSTP1 and associated cancer risks are shown in the next table:

Source: (GSTP1 and cancer: Expression, methylation, polymorphisms and signaling (Review)) 

Therefore, xenobiotics can not only overload the GST detoxification processes, but they can damage GST and the enzymes it encodes, like GSTP1.  Restricting xenobiotics and stress is crucial to keeping this important defense system working optimally!

Here’s a bit more on how these genes detoxify.  Although GST genes do not make glutathione (GSS genes do), they regulate its use.

Glutathione has been described as “the mother of all antioxidants” because it recycles vitamins C and E, which are other antioxidants, and of course it binds and modifies toxins from our environment so that we can get rid of them.  According to Dr. Mark Hyman, “The secret of its (glutathione’s) power is the sulfur (SH) chemical groups it contains. Sulfur is a sticky, smelly molecule. It acts like fly paper and all the bad things in the body stick onto it, including free radicals and toxins like mercury and other heavy metals.” (Glutathione: The Mother of All Antioxidants)

Glutathione (GSH) is a tripeptide molecule consisting of the amino acids glutamate, cysteine, and glycine. It is the most abundant antioxidant in the human body that contains thiol (an organic sulfur compound).  Peptides are chains of 2 to 50 amino acids that are linked together.  For reference, proteins are also chains of amino acids linked together, but these number over 50 and usually more than 100. Here is a visual aid, noting that Glutathione falls under the Peptides category. 

Source: The Difference between Peptides and Proteins

Glutathione exists in two states in cells: reduced (GSH) and oxidized (GSSG).  Oxidized glutathione is actually 2 reduced glutathiones bound together at the sulfur atoms.

Source: Glutathione!

The difference between reduced and oxidized glutathione is that GSH (reduced) is the “recharged” version, while GSSG (oxidized, also called glutathione disulfide) is the “spent” version. GSH is also called “free glutathione” in that it has its sulfur site ready to bind to xenobiotics, while the site on the spent version is not available because it’s stuck to another glutathione molecule. 

The body is constantly recycling glutathione from the oxidized to the reduced version (for more on how this happens, this video is really helpful) .  Healthy cells at rest have a GSH/GSSG ratio >100:1, meaning that there is much more GSH (recharged) available than GSSG (spent).  When cells are exposed to oxidant stress such as xenobiotics, the ratio can drop to 1:10. This is very dangerous, because depletion of GSH and accumulation of GSSG is actually directly toxic to cells, causing their death (apoptosis).  (Glutathione!)  

Glutathione (GSH) production also drops with age and disease. Unfortunately you can’t just “pop a pill” for more GSH (contrary to many medical claims on the internet!). The body, however, can  make its own GSH in the liver with the amino acids cysteine, glutamate, and glycine. The best ways to boost our bodies’ manufacturing of GSH are to eat foods rich in glutathione or its building blocks (amino acids of cysteine, glutamate, and glycine), increasing your intake of vitamin C, and getting enough sleep and exercise.  (10 Natural Ways to Increase Your Glutathione Level)

Here’s a recap about GST, GSTP1, and glutathione:

• GST is the gene responsible for encoding GSTP1.

• GSTP1 is the enzyme that regulates the ability of glutathione to bind to xenobiotics.

• Glutathione is an an enzyme that exists in 2 forms: GSH (reduced) and GSSG (oxidized).

• GSH is also called “free glutathione” and in healthy cells, exists in a 100:1 ratio with GSSG.

• Xenobiotics are those chemicals to which we are exposed that come from outside our bodies.  POPs (persistent organic pollutants) are xenobiotics.

• GSH binds with xenobiotics in the presence of the GSTP1 enzyme.

• GSSG cannot be used to bind xenobiotics, it first must be converted back to GSH.

• Stress and xenobiotics are dangerous in that they can cause changes to GST and GSTP1 which affect their ability to detoxify our bodies, making the body prone to cancer.

• Unchecked stress and xenobiotics also overwhelm free glutathione, causing cell death.

• We can help restore proper glutathione balance and immune function by limiting stress and xenobiotics, eating the right foods for manufacture of GSH and getting enough sleep and exercise.

Breaking down Mycotoxins and mVOCs with Enzymes and Non-Toxic Cleaners

If you haven’t read our white paper on mold, mycotoxins and mVOCs, you should!  While discussing the meanings of these scientific terms with our team, we thought it would be even more helpful to break it down to the vernacular.  Mold is like most other living organisms that excrete waste products.  Mycotoxins are not similar to excrement, in that they are not secreted because of normal growth, development or reproduction of the mold (they are secondary, not primary metabolites).  They are chemicals secreted in offense and defense, and in stressful situations.  Thus, mycotoxins are like sweat–the toxic sweat of mold (yuck!).  They can be sent aloft into the air on mold spores (which are also released whenever the mold is stressed or physically agitated), or even smaller fragments of mold and dust, which are all easily breathed in.  

Microbial Volatile Organic Compou0nds (mVOCs) are gasses as a product of growth, development or reproduction (some are primary metabolites), and as a signal to other microbes around them (secondary metabolites).  This means that mold uses mVOCs to communicate and affect the behavior of other molds around it, even as a competitive tool to directly exert antimicrobial activity (suppressing or eliminating potential enemies). (Volatile affairs in microbial interactions)  As such, mVOCs are akin to body odor–the toxic body odor of mold, which it uses to intimidate other mold!   Although they are meant to signal other microbes, even humans can recognize the smell of some mVOCs in that musty, earthy smell that is a tell-tale sign of mold.

It has been shown that the mycotoxins can be eliminated by various physical means such as thermolysis (destruction by intense heat), radiation treatment and low-temperature plasma (bipolar ionization).  They can also be destroyed by chemical methods such as oxidation (removal of electrons), reduction (addition of electrons), hydrolysis (breakdown by reaction with water), alcoholysis (breakdown by reaction with alcohol), absorption and adsorption, and biological methods by using living things like bacteria or other molds. (Enzymes for Detoxification of Various Mycotoxins: Origins and Mechanisms of Catalytic Action)  Only two  of these methods are permitted to mitigate mycotoxins in foods, however, contamination of raw materials with chemicals and/or products of side reactions limit their use.

Alternatively, using enzymes to detoxify mycotoxins mostly avoids these problems.  First of all, what is an enzyme?   Enzymes are proteins produced by living organisms that act as catalysts in chemical reactions.  Enzymes can either build up or break down.  For our purposes, cleaning enzymes facilitate breaking down microbes and their byproducts that cause sickness, stinkiness or stains.  

Scientists have been exploring making artificial enzymes since the 1990’s, and many of these are mimicking enzymes found in nature.  Here are the most common types of natural enzymes (from Simple Science: How in the World do Enzymes Clean?):

• Proteases break down protein-based soils including blood, urine, food, feces, wine and other beverages.
• Lipases break down fat molecules like oils and grease.
• Amylases break down starch molecules like eggs, sugars, sauces, ice cream, gravy.
• Cellulases are used to soften fabric and restore color to fibers made up of cellulose material. They also remove particulate soil and reduce fabric graying and pilling.

Various enzymes can also be combined to treat the combinations of mycotoxins that are produced by some molds.  For example, cytochromes are enzymes that include a number of compounds consisting of an iron-containing molecule bonded to a protein (cancer.gov).  Cytochromes are usually used within mammals as detoxifying agents of multiple toxic compounds, including mycotoxins.  Cytochromes in the human liver are able to convert aflatoxin B1 (a cancer-causing mycotoxin) into Aflatoxin M1, which is 10 times weaker in carcinogenic potency.  In turn, glutathione s-transferase, another enzyme in our bodies, leads to the excretion of aflatoxins from the body (2016 study).   

Since the enzyme-based approach for degrading mycotoxins in homes is new, there aren’t that many commercial products on the market.  In fact, Green Home Solutions claimed in 2022 that it was the “is the only professional remediation company that combines the ANSI/IICRC standards for mold remediation with a state of the art proprietary disinfectant/ fungicide that not only kills bacteria and molds, but continues to work by breaking down the allergenic protein structures into harmless amino acids.”  It is only available for professional use, but their product description shows that several enzymes are combined for maximum effect on the broad range of mycotoxins that may be found in a home:

• AMYLASE is an enzyme that digests the mold’s outer membrane or cell wall. It dissolves and causes the insides to leak out.
• LIPASE is another enzyme in our product formula which attacks and breaks down the fatty lipids inside of and in between the mold membranes.
• PROTEASE breaks down allergenic proteins at the mold’s core or nucleus, eliminating them from the air you are breathing.

Since we revealed the real nature of mycotoxins and mVOCs (they’re like toxic sweat and body odor), we thought you might like some real solutions you can use to get rid of these toxins in your home!   Elimination of the mold colony is first and foremost, so it’s best to contact an inspector if you can’t find or handle the problem yourself.  Getting rid of the bulk of mold will remove much of the mycotoxins and mVOCs, but mycotoxins can still be present in dust (they are very hardy and indestructible by heat), and mVOCs may be present in absorbent furnishings.  Here are some products and techniques that can rid your home of the remainder of these toxins.

• EC3 Enzyme Cleaner Concentratie is a non-toxic solution also trusted by many mold remediation companies and mold awareness sites, such as moldfreeliving.com.  It is an enzyme solution with citrus and essential oils that is safe for food-prep surfaces, can also be used in the laundry and many surfaces around the home, even as a degreaser. It was proven effective for mold spores and mycotoxins by a 3rd-party, CAP-accredited lab.   

• MycotoxinKlear is recommended for all mold and mycotoxin products in use of a fogger or electrostatic sprayer.  

• The enzyme-based approach for degrading mycotoxins in homes is not common on the commercial scale.  However, some mold remediation companies use them with great results.  The Mold Pros use SurfaceGuard, which was shown in a 2019 study analyzed by RealTime Labs to break down ochratoxin, aflatoxin and gliatoxin to undetectable levels.  Unfortunately SurfaceGuard is only available to commercial remediation companies.

• Most MVOCs can be mitigated with activated carbon filters, because they are in a gas form and can flow through and be adsorbed by the carbon molecules.  You can try our Germ Defender with carbon filters, or purchase carbon filters for your standalone HEPA unit.  Mycotoxins, however, will not be affected by carbon filters because mycotoxins are attached to spores, fragments of spores, and dust, which are not captured by carbon filters.

If you can’t remove all of the mold, the next best thing may be encapsulation.  Encapsulation is a controversial method in that it does not physically remove the mold but surrounds it with a protective layer that does not allow it to release spores or mycotoxins into the environment anymore.  If encapsulation is done properly, though, it can be a safe method that allows homeowners to save wet drywall and wood that have not dried out completely, by sealing any mold that is present and not allowing more to grow.  As demonstrated by EarthPaints, encapsulation either needs to be applied to completely dry substrates, or with a solution that allows the substrate to completely dry over time.  Their Lime Prime paint is a non flammable mineral shield that saturates wood fiber cells and pozzolanically reacts with concrete and gypsum. Encapsulated Substrates dry out properly and in 30-60 days are ready for follow up after a flood. (earthpaint.net)  The problem with mold encapsulation is that it must completely coat surfaces in a space with a durable non-toxic product, and of course the space must be clean and dehumidified, or the mold will “break through” and continue growing as dust and moisture allow it to propagate.  For more information on whether encapsulation is right for a space in your home, this article by a building biologist is very helpful.